Imperial/Cell by Date/Implementation
From 2007.igem.org
< Imperial
Cell by Date: Implementation
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Construct Design | Concept 1 | Concept 2 | |||||
Stage 1: Availability | Found as BBa_I13522 in Registry | Found as BBa_E7104 in Registry. | |||||
Stage 2: Cloning / Amplification | |||||||
Stage 3: Ligation | Not Required | Since BBa_E7104 was not functional, we made our own promoter and and ligated it to BBa_I13504
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Stage 4: Purification of Activator Proteins | Not Required | Not Required | |||||
Stage 5: Quality Control | | | |||||
Stage 6: Final Implementation Status | Ready for Testing | Ready for Testing |
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Chassis Design | ||
Stage 1: Availability | Possibility of making Homemade S30 Cell Extract. | Commercially available S30 Cell Extract purchasable from Promega. |
Stage 2: Preparation | Preparation: Done (Protocol) | Preparation: Done (Protocol) |
Stage 3: Quality Control | ||
Stage 4: Final Implementation Status | Aborted. Expression capacity of chassis was low. | Ready for Testing |