From 2007.igem.org

 

AAG TCTAGA aaagaggagaaa ACTAG                                                              | 5' ext + xba1 +rbs+ spacer
atggacaaattcgacgctaatcgccgcaaattgctggcgcttggtggcgttgcactcggtgccgccatcctgccgacccctgcgtttgca | ycbK TAT signal peptide
atgcggaacgggctcgtgtggctgctgcaccccgcgctgcccggcaccttgcgctccatcctcggcgcccgcccgccgcc           | 5' IDE
acatgactgagttcaagcgtggcctgccactgtttcccctcgtgaaaccacatattaacttcatggctgcaaaactctga           | 3' IDE
tactagtagcggccgctgcag                                                                      | spe1+not1+pst1 site
GAA                                                                                        | 3' extending region

 

1.     TAT_Forward: AAG TCTAGA aaagaggagaaa ACTAG atggacaaattcgacgct

2. IDE_TAT_Reverse: ggctggggacgcaaacgttacgccttgcccgagcac
   

3. IDE_IDE_Forward: ccgacccctgcgtttgcaatgcggaacgggctcgtg 

4.     IDE_Reverse: taccgacgttttgagactatgatcatcgccggcgacgtcctt
   

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IDE Coding Sequence

atgcggaacgggctcgtgtggctgctgcaccccgcgctgcccggcaccttgcgctccatcctcggcgcccgcccgccgcc
cgcgaagcgactgtgtggattcccaaaacagacttacagcacaatgagtaatccggccatccagagaatagaagaccaaa
ttgtcaagtctcctgaagacaaacgggaataccgtggactagagctggccaatggcatcaaagtgcttctcatcagcgat
cccaccacagacaagtcctcagcggccctcgatgtgcacataggttcactgtcagaccctccaaatattcctggcttaag
tcatttttgtgaacatatgctgtttttgggaaccaagaaatatcctaaagaaaatgaatatagccagtttctcagtgaac
atgctggaagttcaaatgcattcaccagtggagaacacaccaattattatttcgatgtttcccatgaacacttggaagga
gccctggacaggtttgcgcagtttttcctgtgccccttgtttgatgcaagttgtaaagacagagaggtgaacgctgtcga
ttcagaacatgagaagaatgtgatgaacgatgcctggagactcttccagctggaaaaggctacggggaaccccaaacacc
ccttcagcaaatttgggacaggaaacaaatatactctagagactcggcccaaccaagaaggcatcgacgtaagggaagag
ctcttgaaatttcactctacgtattattcgtccaatctgatggcgatttgtgttttaggtcgagaatccttagacgacct
cactaacctggtggtgaagctgttctccgaggtggagaacaagaacgttcctctgcctgagttccctgagcaccctttcc
aggaagagcatctcaggcaactttataaaatagtacccattaaggatattagaaatctttatgtgacatttcccatacca
gaccttcagcagtactacaaatccaatcctggtcattatcttggtcatctaattgggcacgaaggtcctggaagcctgtt
gtcagaactcaaatcaaagggctgggtgaataccctggttggtggacagaaggaaggagcccgaggttttatgtttttta
tcattaatgtggacttaactgaagaagggttattgcacgttgaagatataattttgcacatgtttcaatacattcagaag
ctacgtgcagaaggacctcaagaatgggttttccaagagtgcaaggacttgaatgctgttgctttcagatttaaagataa
agagagaccacgaggctatacgtccaagattgcaggaaagttgcactattatcccctaaatggagtgctcacagctgaat
atctattggaggaatttagacccgacttgatagacatggttcttgataaactcagaccagaaaatgtgcgggtggcaata
gtttccaaatcatttgaagggaaaactgaccgcacagagcagtggtatggaacacagtataagcaagaagctatcccaga
ggacatcattcagaaatggcaaaatgctgaccttaatggaaaatttaagcttccaacaaagaatgaatttattcccacaa
attttgagattttgtcattagaaaaagatgcaacaccataccctgctcttattaaggatacagccatgagtaagctgtgg
ttcaaacaagatgataaatttttcttgccgaaagcttgtctcaactttgaatttttcagcccgtttgcttatgtggaccc
cttgcactgtaacatggcctatttgtaccttgagctcctcaaagactcactcaacgagtatgcatatgcagcagagctag
caggcctgagctatgacctccaaaataccatctatgggatgtatctctcagtgaaaggttacaatgacaaacagccaatt
ttgctgaagaagatcaccgagaaaatggctacttttgagattgataagaagagatttgaaattatcaaagaggcctatat
gcgatctcttaataacttccgggctgagcagcctcaccagcatgccatgtactacctccgcttgctgatgactgaagtgg
cctggactaaagatgagctaaaagaagcccttgacgatgttaccctcccccgacttaaggccttcatacctcagctgctg
tcacggctgcatattgaagcacttctccatggaaacataaccaagcaggctgccttaggagttatgcagatggtagaaga
cactcttatcgagcatgctcacaccaaacctctccttccaagtcagctagtccggtacagagaagtccagctccctgaca
gaggatggtttgtttatcagcagaggaatgaagttcacaataactgcggcatcgagatctactaccagacagacatgcag
agcacctcggagaacatgttcctggagctgttttgccagattatctcggagccttgcttcaataccctgcgcaccaagga
gcagcttggctatattgtcttcagtggaccccgtcgggccaacggtatccaaggcttgcgcttcatcatccagtcagaaa
aaccaccccactacctggagagcagagtagaagccttcttgattaccatggaaaaggccatagaggacatgacagaggag
gcgttccaaaaacacattcaagcactagcgattcggcgactcgacaaaccaaagaaactctctgcggagtgtgctaaata
ctggggggaaatcatctcgcagcagtacaattacgacagagataacatagaagttgcatatttaaagacacttaccaagg
acgatatcatcagattctaccaggaaatgttggctgtcgacgcaccgaggagacataaagtgtctgtccatgttcttgcc
agggaaatggattcttgtcctgttgttggagagttcccctctcagaatgatataaacttgtccgaagcgccacccttgcc
acaacctgaagtgattcacaacatgactgagttcaagcgtggcctgccactgtttcccctcgtgaaaccacatattaact
tcatggctgcaaaactctga