PCR HNX-F and HNX-R on H-Nox   (567bp, BglII/XhoI) 
Sub into pBca1144              ( BglII/XhoI, 2054+910  L) 
Product is pBca1144-H-NOX
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HNX-F Forward primer anneals to BglII ctagtAGATCTATGAAGGGGACAATCGTCGG

HNX-R Reverse primer anneals to XhoI cgtgaCTCGAGgaatgGGATCCTTAATTTTTCTTATACTCA