From 2007.igem.org

Biobrickificreation of pir I716102 (R6K Origin Inducer)
---------------------------------------------------
PCR pir-F/pir-IR on pir116		(375 bp, gp = A)
PCR pir-IF/pir-R on pir116		(599 bp, gp = B)
---------------------------------------------------
PCR pir-F/pir-R on A+B			(949 bp, BglII/XhoI)
Sub into pBca9145-Bca1144		(BglII/XhoI, 2063+910, L)
Product is pBca9145-I716102-pir
---------------------------------------------------
dt001-pir-F   Forward Cloning of pir   ccaaaAGATCTatgagactcaaggtcatgatg
dt002-pir-IF  Forward Removing the BglII site in pir  gaagagttagaCcttaacattattg
dt003-pir-IR  Reverse Removing the BglII site in pir  caataatgttaagGtctaactcttc
dt004-pir-R   Reverse Cloning of pir   gctagCTCGAGttaGGATCCtcaccccttagcttttttgg