From 2007.igem.org

Construction of hemA basic part from E.Coli CFT073
PCR kd013/kd016 on hemA	(547bp, gp=A)
PCR kd015/kd014 on hemA	(761bp, gp=B)
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PCR kd013/kd014 on A+B 	        (1288bp, Bg1II/XhoI)
Digest pBca9145-Bca1144#5  	(BglII/XhoI, 2063+910, L)
Product is pBca9145-I716152
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kd013	Forward Bg1II for hemA		ccaaaAGATCTatgacccttttagcactcggtatc		
kd014	Reverse Xho1 for hemA	    	gctagCTCGAGttaGGATCCctactccagcccgaggctgtc
kd015	Forward remove the Bg1II site in hemA    	gcgcggcAGATTTttgaatc
kd016	Reverse remove the Bg1II site in hemA    	gattcaaAAATCTgccgcgc