Spherical Multicellular Organism!?
When you see a shape in Nature, you will notice whether a sphere, which is absolutely symmetric in 3D, is really stable or not in Nature. In fact, an oil droplet is a sphere in water. Red blood cell in a hypotonic solution shows its shape change to a spherical balloon. However multicellular organisms have their shape different from a sphere except Marimo. Of course, other algae do not show spherical shape, they live on a surface of stone. It is quite intriguing how Marimo remains its spherical shape in a lake!
Our team focuses understanding how such spherical structure can be sustained even when it Is multicellular organisms.
- Affinity Tag.
- Communication Module.
- Size Control.
Two cells are used in our system: AHL senders and receivers. Senders generates the affinity tags constitutively, while receivers generates them only when they are induced by AHL. The marimo-forming goes like this:
- Make the sender core by sticking with the affinity tag.
- Insert the sender core into the receiver culture.
- The sender core produces AHL, which make the near receivers to generate the affinity tags and GFPs.
- The affinity tagged receiver sticks with the central sender core. This will continue until the AHL cannnot reach the marimo boundary,
- When the AHL reached the marimo boundary, the adsorping stops, which makes a finite-sized marimo bacteria.
- Make the affinity tag by inserting the his-tag into the flagellar fillament.
- Making Receivers
- Making Senders
- Improve Sender
- Improve Receiver
- Localize AHL
Although we could not reach them, below we describe the goal that we set to make a marimo bacteria.
- A test of adsorption between flagella
- A test to confirm a limit of size
- A test to form Marimo
- A test of size control
See here : our brainstorming of the marimo future!