We aimed to make a spherical gathering of bacteria such like marimo by ordering bacteria go get together and stick to each other.
What our system requires
Make a His-tagged Flagella. We aimed to stick bacteria by displaying histidines (which bonds each other through metal ions) on the flagellar filament.
Make a Bacterial Communication. We make 2 types of cell(sender&receiver) having different gene circuit. Senders sticking each other in advance send a signal to receivers and receivers grow affinity tags.
Make an AHL localized region for quorum sensing.
How Our System Works
- Senders whose flagella display the histidine tags stick to each other through metal ions. This becomes the core of Bacteria Marimo. The senders also produce AHL to sign the receiver cells.
- Receivers express his/flagella and GFP in high [AHL]; only when they get close to the senders core.
- Receivers stick to the senders core and themselves through metal ions.... one after another.
- As seen, the cluster grows like a snowball. At the same time, the receivers degrade AHL and thus AHL diffusion space around the mixed cluster limited. By controlling the rate of AHL degradation and so on, one can define the size of such Bacteria Marimo.
For other strategies to make marimo, visit the official website of iGEMCHIBA