From 2007.igem.org

Contents 1 Week1(8/16~8/23) 2 Week2(8/24~8/31) 3 Week3(9/1~9/8) 4 Week4(9/9~9/15) 5 Week5(9/16~9/22) 6 Week6(9/23~9/29) 7 Week7(9/30~10/6)

Week1(8/16~8/23)

Monday,August,17,2007

• pUC-FliCのPCR

F/R	FliC-207	FliC-228	FliC-240	FliC-252	FliC-267
FliC-207	①
FliC-228	②	③
FliC-240	④	⑤	⑥
FliC-252	⑦	⑧	⑨	⑩
FliC-267	⑪	⑫	⑬	⑭	⑮

Recipe(単位：μ)	①	②	③	④	⑤	⑥	⑦	⑧	⑨	⑩	⑪	⑫	⑬	⑭	⑮
pUC19-FliC	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1
FliC-207F	5
FliC-207R	5	5		5			5				5
FliC-228F		5	5
FliC-228R			5		5			5				5
FliC-240F				5	5	5
FliC-240R						5				5			5
FliC-252F							5	5	5	5
FliC-252R										5				5
FliC-267F											5	5	5	5	5
FliC-267R															5
10×Buffer	10	10	10	10	10	10	10	10	10	10	10	10	10	10	10
dNTPmix	10	10	10	10	10	10	10	10	10	10	10	10	10	10	10
Vent	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1
dH2O	68	68	68	68	68	68	68	68	68	68	68	68	68	68	68
Total	100	100	100	100	100	100	100	100	100	100	100	100	100	100	100

Week2(8/24~8/31)

Week3(9/1~9/8)

Week4(9/9~9/15)

• Swaming test

1. FliC-267His-RBSプラスミドをJW1908(KEIO⊿FliC)にTransformation（Positive Control=ASKA,Negative Control=KEIO-JW1908にFliC-267HisをTransformしたもの）
2. LB培地で37℃12h2ml培養
3. トリプトン培地（Agar0.25%）の中央に1μinoculate
4. 30℃、24-48h,Taxis
5. Taxis ling を観察

• Sample

Week5(9/16~9/22)

Week6(9/23~9/29)

Week7(9/30~10/6)