June 15

From 2007.igem.org

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  • pLac cfp inoculated in 3ml LB+3ul amp and K12Z1 in 3ml LB+3ul spec.
  • Dilutions were made in 3ml M9.
  • Same dilutions made for FACS (1 ml).
  • pLac cfp + K12Z1 induced with IPTG conc (0,1,10,50,100,1000 uM) in sets of two.
  • Also,0 and100 uM IPTG dilution were made to check OD (two sets)
  • The new constructs were transformed.