June 17

From 2007.igem.org

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Experiments

  • pLac yfp was inoculated in 3ml LB+3ul amp and K12Z1 in 3ml LB+3ul spec.
  • pLac luxI cfp was inoculated in 3ml LB+3ul amp, K12Z1 in 3ml LB+3ul spec.
  • IPTG induction for both pLac yfp and pLac luxI cfp was done for 0,1,10,50,100,1000 uM concentration along with K12Z1 as control.
  • pLac cfp could not be imaged due to lack of pelletes.