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Experiments

For [C]

• The inductions were done in 50 mL Glu M9 in 250 mL flask with 5 ng/mL conc of aTc and 1,0.1 uL/mL of the inoculum. (5*50 ng=0.25 ug == 25 uL of 10 ug/mL aTc)

• pLLuxR.Y.pR.C and K12z1 were inoculated in LB.

• Filter required OD flask, store at 4 degrees.

• Seven dilutions for pL.LuxR.Y.pR.C were made with 50 old:50 new 2xM9 along with -ve control: K12Z1.

For [D]

• The inductions were done in 50 mL Glu M9 in 250 mL flask with 1 ng/mL conc of aTc and 1,0.1 uL/mL of inoculum. (1*50 ng=0.05 ug == 5 uL of 10 ug/mL aTc).

• pLluxR.Y.pR.C and K12z1 were inoculated in LB.

• The flask containing the required OD was selected and was stored at 4 degrees.

• Seven dilutions for pL.LuxR.Y.pR.C with 50 old:50 new 2xM9 along with -ve control: K12z1 were made.

Microscopy

• Open Loop samples [B] were imaged.

Analysis