From 2007.igem.org

iGEM training list

Space

• Bench use - generally Frank and Kevin's, borrow others upon request
• Desk space - use Ingalls

Glassware

• clean and dirty dishes
• autoclave tape, foil

Gels

• Gel equipment: agarose, precast, acrylamide, E-gel
• Gel buffers, dyes, wax paper, loading/mixing tricks, etc.
• Gel doc system
• Blue light system for cutting gels
• darkroom

Chemicals

• Inventory management system
• Weighing materials, chemicals, inventory
• Chemical hood - what mix there?

Consumables

• miniprep kits
• enzymes
• sterile pipettes
• Growth media, agar
• Plates, tips, etc.
• Agarose
• chemicals

Safety

• Waste locations
• sharps, pipettes, razors, needles, biohazard
• lab coats
• eyewear
• burners

Storage of materials

• Non-volatile, room temp stuff
• Acids and bases
• Alcohols
• Flamables, volatiles
• Small fridges, big fridge, -20 freezer, -80 freezer (what goes where)
• -80 Freezer organization
• coldroom

Ordering

• procedure
• packing slips

Sequencing, oligos, enzymes

• Agencourt
• NEB, enzyme inventory
• IDT

Equipment

• manuals
• Thermocyclers
• incubators
• centrifuges
• autoclave
• pH calibration
• nanodrop
• plate reader

Software

• nanodrop
• plate reader
• clone manager
• affy system
• database

Core facilities

• Room 623 - ice, gel doc, lyophilizer, extra incubator, coldroom
• Flow cytometer
• Microscopy
• RT PCR