McGill/Results/Method 1



Using a spectrophotometer, we were able to achieve more precise quantitative results for the oscillating network and we were also able to test many samples at a time with variations in the testing conditions:

  • variable concentrations of cells
  • variable concentrations of IPTG
  • Dox (tetracycline analog)
  • AHL (synthetic auto-inducer).

Oscillations shown on a plate reader for the J40001 experiment - conclusion: BL21 cells endogenously express Lac