Melbourne/Gvp site dirrected primers

From 2007.igem.org

Name Sequence: 5’-…..-3’ %GC Tm Length posn
GvpL-g213a GGATATGAGCTGGCTGCAAGAAAAAGCATTTCATCATCACG 44 83 41 19
GvpL-g213a_R CGTGATGATGAAATGCTTTTTCTTGCAGCCAGCTCATATCC
GvpL-g318a GGTGAAGAAAGTCTGCAAGCAGCTATTGAGATTAAC 42 80 36 18
GvpL-g318a_R GTTAATCTCAATAGCTGCTTGCAGACTTTCTTCACC
GvpL-g351a CAAAGAAAAGATAGAAAATTCACTGACGCTGCTTCAAGG 38 80 39 16
GvpL-g351a_R CCTTGAAGCAGCGTCAGTGAATTTTCTATCTTTTCTTTG
GvpL-g696a CGTGTTTCTTCTCCCGTCTCTGCAAATTACTAAGTTCGTAAACG 43 84 44 25
GvpL-g696a_R CGTTTACGAACTTAGTAATTTGCAGAGACGGGAGAAGAAACACG
GvpA-t57c GCAGAAGTGATTGATCGAATCCTCGACAAAGGGATTGTCATCG 47 85 43 21
GvpA-t57c_R CGATGACAATCCCTTTGTCGAGGATTCGATCAATCACTTCTGC
GvpP-g441a CGACCAGCTGCAACGCATTGAAGAGATGC 55 81 29 13
GvpP-g441a_R GCATCTCTTCAATGCGTTGCAGCTGGTCG
GvpQ-g150a GGGAAACTGCAAGAAAAAGCAAATGAAGCGTCAGAAAAACTGC 42 83 43 12
GvpQ-g150a_R CAGTTTTTCTGACGCTTCATTTGCTTTTTCTTGCAGTTTCCC
GvpQ-g183a GCGTCAGAAAAACTGCAAGAAACAAAAGAAAAAAATGCCC 38 80 40 18
GvpQ-g183a_R GGGCATTTTTTTCTTTTGTTTCTTGCAGTTTTTCTGACGC
comp-g318a ttataaaggtgaagaaagtctgcaagcagctattgagattaacaaag 32 80 47 26
comp-g318a_R ctttgttaatctcaatagctgcttgcagactttcttcacctttataa
Jrmy-g318a GCAAGCAGCTATTGAGATTAACAAAGAAAAGATAGAG 35 59.1 37 4
Jrmy-g318a_R GCTGCTTGCAGACTTTCTTCACCTTTATAAATG 39 59.7 33 6