Melbourne/Ligation Protocol


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  • Applications: Ligate DNA fragments together

  • Time to complete protocol: Overnight, 2 hours, or 10 minutes
    • Lab time: 5 minutes
    • Waiting time: Overnight, 2h, 10min
  • Approximate cost of materials: $


Method from primary and secondary reagents

Primary & secondary Reagents Required including controls
Method including controls

Add to a sterile 1.7ml Eppendorf

Incubate Overnight (if using 10X buffer) at 4 degrees, 2h or 5min (if using 2X buffer) at room temprature.

Equipement Required