Melbourne/meeting 19 July 2007

From 2007.igem.org

  • Alisa: Status report on experimental work:
  • Phil Status report on gas vesicles and gas vesicle plans:
    • currently have cells growing on plates - no checks done yet.
    • Plan up to Biobricks of basic parts outlined at 9 weeks including US shippment.(we have 13 max).
    • Details complete up to end of mutation for pNL29 but not pNL26 using primers designed by stratagene program. Urgent for .
    • Some items require ordering,round bottom falcon tubes, or manufacture NZY broth, blue/white plate.
    • Lac promotor and antibiotic death plasmids required.
    • Primers on critical path -------phil to complete ASAP.
  • Jan Status report on Blue light sensor and light box apperatus, lead time on DNA fabrication?
    • Plan to have biobrick fabricated (up to 3Kb on fast turnarround= 525USD.)
  • Adhesion: to be abbandoned for this year, unless clever idea comes to mind.
  • Phil:Status report documentation wiki:
    • synopsis: A lot to be done, -------Kasia to help.
    • Priority should be on plans and experimental report analysis and conclusions-----Alisa .
    • If only Pat & Alisa doing lab work not much documentation needed.
    • Equipement:
      • MissingLas3000 method (in lab notebook)
      • Missing DNA measurement protocol (eppendorf)
    • Reagents pages incomplete:
      • Some location pictures not finished or uploaded.
      • Some newer reagents not entered.
    • Secondary reagents pages not made:
      • LB, Plates, TAE , NZY+ Broth
    • Protocol part:
      • No connections from Protocols to reagents.
      • Ligation protocol missing
    • Lab notebook:
      • Format uses too many lines to see connections between days.
      • Latter part missing links
      • Can table of contents be restricted to date level?
    • Experimental reports:
      • Lacks discussion/conclusion
      • Ligation experiment missing
      • Lattest few gells not marked up and entered.
    • Project overview link broken
    • Background:
      • GFP missing ,
      • Red photo sensor to GFP test harness missing.
    • Plan: Only Gas vesicles plan entered.


  • Phil: Admin timetable: 13wks to go.
    • 1 August Team project descriptions due (Late summer teams)
    • 1 September Final team rosters due
    • 1 September Jamboree attendance fees due $100USD per member + 225USD for others.
    • 26 October Project and part documentation due DNA recieved USA
    • 26 October BioBrick Part DNA received by the Registry
    • 3-4 November iGEM Competition Jamboree, MIT, USA
  • ALL:Individuals desired time committment? (outstanding)

(eg 12hrs per week equiv to an extra subject, 3 hrs on wed afternoon only)

  • Desire to travel/ present at MIT in November?
  • Timetabling. Jan to complete.
  • Discussion:
    • What can we reasonably expect to achieve? Not adhesion. Reframe as two year project- parts this year.
    • Jan keen to go to MIT to present, no one else particularly due to exam timetable.
  • Synthesis details:
    • 30Kb per month 100kb total all teams divided equally 57 teams until 31 July(2Kbp max).
    • USD 25c per bp. 75c beyond that .
    • maximum construct length 1kbp sub cloned into geneart standard vector.
    • lead time 12 days plus shipping say 3weeks total.
    • standard 8day fast track plus shipping 2 weeks 100bp USD435 , 3000bp USD 525.


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