NYMU Taipei/Lab Notes/2007 9 19

From 2007.igem.org

  • GEL re-check for INS_A PCR annealing temperature gradient (load 10 uL, 2 uL dye)
GEL checkImageJ check at 122 bp
NYMU Taipei 20070919 INS A PCR GEL check.jpg NYMU Taipei 20070919 INS A PCR plot check.jpg
  • lanes from left to right
    • 100 bp ladder, 49.8 °C, 52.3 °C, 55.5 °C, 58.7 °C, 60.9 °C, 55.5 °C (control with template)
  • ligation for (CinR+HSL) + D-term
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