NYMU Taipei/Lab Notes/2007 9 30

From 2007.igem.org

PCR for TAT_A-INS_A TAT_B-INS_B

size Primer Template
1 TAT_A-INS_A 209 TAT_F+INS_A_R TAT_A,INS_A PCR product@9/29
2 TAT_B-INS_B 233 TAT_F+INS_B_R TAT_B,INS_B PCR product@9/29
Template1 1uL
Template2 1uL
10X buffer 5uL
2.5mM dNTP 2uL
Primer_F 1.5uL
Primer_R 1.5uL
Pfu polymerase 0.5uL
ddH2O 37.5uL
Total 50uL
Step Temp (°C) Time (mins) Cycle
Initial Denaturation 95 10
Denatuation
Annealing
Extension
95
55
72
1
1
1
35
Final Extension 72 10
Soak 4




2%Gel check for TAT_A-INS_A TAT_B-INS_B

NYMU Taipei 20070930 TATA-INSA check.jpg

  • Well 1 TAT_A-INS_A 10uL
  • Well 2 TAT_B-INS_B 10uL
  • Well 3 100bp ladder 5uL