OtsB-I716302

From 2007.igem.org

PCR otsBF/otsBIR on otsB (485 bp, gp=A) PCR otsBIF/otsBR on otsB (381 bp, gp=B)

PCR otsBF/otsBR on A+B (832 bp, BglII/XhoI) Digest pBca9145-Bca1144 (BglII/XhoI, 2054+910, L) Product is pBca9145-BBa I716302

otsBF Forward BglII site anneals to otsB cgtaaAGATCTATGACAGAACCGTTAACCGA otsBIF Removes internal BglII site GCAACGTATTACTCAGATtTGGCCACAAATGGCG otsBIR Removes internal BglII site CGCCATTTGTGGCCAaATCTGAGTAATACGTTGC otsBR Reverse XhoI site anneals to otsB cgtatCTCGAGttaGGATCCTTAGATACTACGACTAAACG

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