Paris/October 10

From 2007.igem.org

yesterday -- tomorrow

Contents

PCR on Colony

  • L52 (O18 & O7) ---> give nothing
  • L53 (O18 & O7) ---> give nothing
  • L55 (O18 & O9) ---> clone 14 & 16 OK

Preparing competent FX85 cells for Recombination Rate Test

  • 1- Classic protocol for preparing TSS chemically competent bacteria
  • 2- Classic transformation protocol (1┬ÁL of Miniprep), except that we don't use an expression period before starting to put the antibiotics (Kan and Amp)
  • 3- We

Recombination Rate Test

Culture of FR781 for FACS