Rapid Assembly of Complex Parts

From 2007.igem.org

Because of the need to rapidly create our constructs (we started building constructs in the middle of August), the incompatibility of many of our sequences with standard Biobrick restriction enzyme sites, and the relative dearth of relevant Biobrick parts, we used PCR overlap assembly technology to assemble our parts all at once, instead of using the stepwise Biobrick method.