Tokyo/Experimental precision and accurecy

From 2007.igem.org

AHL assay

Objective:

To see how precisely we determine the fluorescence from GFP by removing LB media.

Samples:

placQI + GFP in pTrc99A (pos. con.)
A4 ΔP+ GFP (neg. con.)

Procedure:


AHL assay Standard protocol
Make 200 ul of mixtures of pos. con. and neg. con.
The mixture ratios of pos. con. and neg. con. are
(100:0), (70:30), (50:50), (30:70), (10:90), (3:97), (1:99)
Wash

Result & conclusion:

In proportion to the percentages of GFP expressing cells, fluorescence was increased as shown in Fig. 1.
This indicates that the "wash" process well removes the other factors influencing the fluorescence.

"Back to Preliminary assays"