Sorry, there are not enough hands to write the diary.
The last day
Ma Rui is in Shanghai enjoying the convenience of Remote Cooperative Office on the Internet, but suddenly that district (the kernel area of Shanghai) lose its power supply and his documents would not be uploaded to our Wiki. What an unusual accident!
Hard documenting ......
Draft of our schedule for the Jamboree.
The second scheme of NAND gate has been built up and the rough data show that it works. More precise quantitative data is to be measured.
Double-reporters system version 2 has passed sequencing, and been confirmed by X-gal assay and fluorescence observation.
The fluorescent reporter (GFP-aav) in double-reporters system does not work. This fact was confirmed by Ma Rui using several times of fluorescence assays. New version is under construction for next step of NAND gate, and these days Ma Rui have to use classical ONPG method to determine data for designing NOR gate.
Most members of the co-repression promoter family show that they can be repressed by either designed repressor. This result shows that NOR and NOT gates have been got, but no one is suitable for NAND gate. We are considering to modify our scheme to search NAND gate.
The E.Coli cultured from the blue colony shows only very weak green fluoresce intensity. We are considering applying some high copy-number plasmid where the double genes are held.
There is a mistake in my preparing X-Gal solution. Please rework your correlative experiments. Sorry! (by Ma Rui)
First designed repressors. Good job, Bo and Xiaofeng!
Struggling for the final-examinations!
Tianjin TTT workshop
Primary experiments begin around this day.
Finally fix on the project: Biologic Logic Gates.
Tianjin Synthetic Biology and iGEM Workshop
The New Semester Begins !