- There are no colonies for I763010 and C0012. We can't amplify these biobricks.
- J04631 digestion.
- Band extraction.
- R0051+P0412 (I763010) ligation.
- A new R0051+P0412 (I763010) ligation with a new protocol (3h 15°C and 1.2ul T4 Ligase).
- R0051, I763012 digestion with Spe/Pst1.
- P0412, J04631 digestion with Xba/Pst1.
- Run and extraction from gel.
- Transformation of R0051+P0412 (I763010) ligations.
- We plan new amplifications.