From 2007.igem.org

Endonuclease Digestion

• 10uL ddH₂O
• 5uL DNA
• 2uL 10x buffer
• 2uL 1:10 dilution of BSA soln
• 0.5uL Enzyme 1
• 0.5uL Enzyme 2

Incubate at 37C for 2-4 hours

Use this website for restriction enzyme and this site for checking buffer compatibility.