From 2007.igem.org

Using sterile technique:

1. Load 4mL of LB+Amp broth into culture tubes

2. Use sterile stick and dab the desired bacterial colony from your plate

3. Swirl stick in culture tube, ~5 sec.

4. Flame culture tube before closing

5. Place culture tube in 37C shaker for 18-20 hrs.

Flame stick container and LB+Amp broth flask when done.