Paris/July 21
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Eismoustique (Talk | contribs) |
Eismoustique (Talk | contribs) (→Digestion reactions) |
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| style="background: #ccffcc;" |D13 | | style="background: #ccffcc;" |D13 | ||
|<bbpart>mRFP</bbpart> | |<bbpart>mRFP</bbpart> | ||
- | |<bbpart> | + | |<bbpart>pJ23107</bbpart> (MP2.2, MP2.3 & MP2.4) |
|SpeI | |SpeI | ||
|PstI | |PstI | ||
Line 66: | Line 66: | ||
|PstI | |PstI | ||
|pSB1AK3 open vector for BioBrick cloning | |pSB1AK3 open vector for BioBrick cloning | ||
+ | |- style="background: #cccccc;" | ||
+ | | style="background: #ccffcc;" |D16 | ||
+ | |pSB1A2 <bbpart>E0422</bbpart> vector | ||
+ | |<bbpart>E0422</bbpart> (MP6.1 & MP6.2) | ||
+ | |EcoRI | ||
+ | |PstI | ||
+ | |pSB1A2 open vector for BioBrick cloning | ||
+ | |- style="background: #cccccc;" | ||
+ | | style="background: #ccffcc;" |D17 | ||
+ | |BB dig. lox66DapAE.coli | ||
+ | |lox66-DapAE.coli P5 PCR product | ||
+ | |EcoRI | ||
+ | |PstI | ||
+ | |PCR product digested for cloning as a BioBrick | ||
+ | |- style="background: #cccccc;" | ||
+ | | style="background: #ccffcc;" |D18 | ||
+ | |BB dig. lox66DapAsubtilis | ||
+ | |lox66-DapAsubtilis P6 PCR product | ||
+ | |EcoRI | ||
+ | |PstI | ||
+ | |PCR product digested for cloning as a BioBrick | ||
+ | |- style="background: #cccccc;" | ||
+ | | style="background: #ccffcc;" |D19 | ||
+ | |fw insert lox66-DapAE.coli | ||
+ | |lox66-DapAE.coli P5 PCR product | ||
+ | |XbaI | ||
+ | |PstI | ||
+ | |PCR product digested for usage as a forward insert | ||
+ | |- style="background: #cccccc;" | ||
+ | | style="background: #ccffcc;" |D20 | ||
+ | |fw insert lox66-DapAsubtilis | ||
+ | |lox66-DapAsubtilis P6 PCR product | ||
+ | |XbaI | ||
+ | |PstI | ||
+ | |PCR product digested for usage as a forward insert | ||
|} | |} |
Revision as of 19:15, 21 July 2007
Digestion reactions
Digestion Products | |||||
---|---|---|---|---|---|
Number | Product Name | Matrix Name | Enzyme 1 | Enzyme 2 | Description |
D9 | pJ23100 ES vector | pJ23100 (MP1.1 & MP1.2) | EcoI | SpeI | Vector with the J23100 promoter ready for insertion of a RBS/CDS backward-insert |
D10 | pJ23100 promoter fw-insert | pJ23100 (MP1.1 & MP1.2) | EcoI | SpeI | J23100 promoter ready for usage as a fw-insert |
D11 | pJ23107 ES vector | pJ23107 (MP2.1, MP2.2, MP2.3 & MP2.4) | EcoRI | SpeI | Vector with the J23107 promoter ready for insertion of a RBS/CDS backward-inser |
D12 | pJ23107 promoter fw-insert | pJ23100 (MP2.1, MP2.2, MP2.3 & MP2.4) | EcoRI | SpeI | J23107 promoter ready for usage as a fw-insert |
D13 | mRFP | pJ23107 (MP2.2, MP2.3 & MP2.4) | SpeI | PstI | mRFP RBS/CDS/T sequence, can it be used as a backward inser? |
D14 | Cre ORF | J61047 MP9.1 & MP9.2 | XbaI | PstI | Cre ORF extracted for usage as a bw-insert |
D14 | B0030 bw-vector | B0030 MP5.1 & MP5.2 | SpeI | PstI | bacward vector for cloning a CDS bw-insert after the B0030 RBS |
D15 | pSB1AK3 vector | B0015 MP3.1 & MP3.2 | EcoRI | PstI | pSB1AK3 open vector for BioBrick cloning |
D16 | pSB1A2 E0422 vector | E0422 (MP6.1 & MP6.2) | EcoRI | PstI | pSB1A2 open vector for BioBrick cloning |
D17 | BB dig. lox66DapAE.coli | lox66-DapAE.coli P5 PCR product | EcoRI | PstI | PCR product digested for cloning as a BioBrick |
D18 | BB dig. lox66DapAsubtilis | lox66-DapAsubtilis P6 PCR product | EcoRI | PstI | PCR product digested for cloning as a BioBrick |
D19 | fw insert lox66-DapAE.coli | lox66-DapAE.coli P5 PCR product | XbaI | PstI | PCR product digested for usage as a forward insert |
D20 | fw insert lox66-DapAsubtilis | lox66-DapAsubtilis P6 PCR product | XbaI | PstI | PCR product digested for usage as a forward insert |