BerkiGEM2007-SamanthaConstructionFile9

From 2007.igem.org

(Difference between revisions)
Line 1: Line 1:
-
 
'''[rbs][Cre]''' <br>
'''[rbs][Cre]''' <br>
-
'''rbs variants Bca1090''' <br>
+
'''rbs library - want a weak rbs actually''' <br>
-
'''2 Different Cre variants (212 and 213)''' <br>
+
'''3 Different Cre variants (ATG Bca1117, GTG I716212 and TTG I716213)''' <br>
----
----
 +
 +
'''ATG Cre variants''' <br>
 +
 +
Digest pBca1121-Bca9230  (EcoRI/BamHI, 2927+955bp, Small) <br>
 +
Digest pBca9145-Bca1117  (EcoRI/BglII, 3101+9bp, Large)<br>
 +
Plate on CamAmp
 +
Product is pBca9145-I716224. <br>
 +
 +
Digest pBca1122-Bca9230 (EcoRI/BamHI, 2927+1087bp, Small) <br>
 +
Digest pBca9145-Bca1117 (EcoRI/BglII, 3101+9bp, Large) <br>
 +
Plate on KanAmp
 +
Product is pBca9145-I716225. <br>
 +
 +
 +
 +
'''GTG Cre variants''' <br>
 +
 +
Digest pBca1121-Bca9230  (EcoRI/BamHI, 2927+955bp, Small) <br>
 +
Digest pBca9145-I716212  (EcoRI/BglII, 3101+9bp, Large)<br>
 +
Plate on CamAmp
 +
Product is pBca9145-I716224. <br>
 +
 +
Digest pBca1122-Bca9230 (EcoRI/BamHI, 2927+1087bp, Small) <br>
 +
Digest pBca9145-I716212 (EcoRI/BglII, 3101+9bp, Large) <br>
 +
Plate on KanAmp
 +
Product is pBca9145-I716225. <br>
 +
-
Digest pBca9145-Bca1090 (BamHI/BglI/AlwNI, 942+596+553bp, Large) <br>
+
'''TTG Cre variants''' <br>
-
Digest pBca9145-I716212 (BglII/BglI, 2187+923bp, Small) <br>
+
-
Product is pBca9145-I716217. <br>
+
 +
Digest pBca1121-Bca9230  (EcoRI/BamHI, 2927+955bp, Small) <br>
 +
Digest pBca9145-I716213  (EcoRI/BglII, 3101+9bp, Large)<br>
 +
Plate on CamAmp
 +
Product is pBca9145-I716224. <br>
-
Digest pBca9145-Bca1090  (BamHI/BglI, 942+596+553bp, Large) <br>
+
Digest pBca1122-Bca9230 (EcoRI/BamHI, 2927+1087bp, Small) <br>
-
Digest pBca9145-I716213   (BglII/BglI, 2187+923bp, Small)<br>
+
Digest pBca9145-I716213 (EcoRI/BglII, 3101+9bp, Large) <br>
-
Product is pBca9145-I716221. <br>
+
Plate on KanAmp
 +
Product is pBca9145-I716225. <br>

Revision as of 18:54, 20 June 2007

[rbs][Cre]
rbs library - want a weak rbs actually
3 Different Cre variants (ATG Bca1117, GTG I716212 and TTG I716213)


ATG Cre variants

Digest pBca1121-Bca9230 (EcoRI/BamHI, 2927+955bp, Small)
Digest pBca9145-Bca1117 (EcoRI/BglII, 3101+9bp, Large)
Plate on CamAmp Product is pBca9145-I716224.

Digest pBca1122-Bca9230 (EcoRI/BamHI, 2927+1087bp, Small)
Digest pBca9145-Bca1117 (EcoRI/BglII, 3101+9bp, Large)
Plate on KanAmp Product is pBca9145-I716225.


GTG Cre variants

Digest pBca1121-Bca9230 (EcoRI/BamHI, 2927+955bp, Small)
Digest pBca9145-I716212 (EcoRI/BglII, 3101+9bp, Large)
Plate on CamAmp Product is pBca9145-I716224.

Digest pBca1122-Bca9230 (EcoRI/BamHI, 2927+1087bp, Small)
Digest pBca9145-I716212 (EcoRI/BglII, 3101+9bp, Large)
Plate on KanAmp Product is pBca9145-I716225.


TTG Cre variants

Digest pBca1121-Bca9230 (EcoRI/BamHI, 2927+955bp, Small)
Digest pBca9145-I716213 (EcoRI/BglII, 3101+9bp, Large)
Plate on CamAmp Product is pBca9145-I716224.

Digest pBca1122-Bca9230 (EcoRI/BamHI, 2927+1087bp, Small)
Digest pBca9145-I716213 (EcoRI/BglII, 3101+9bp, Large)
Plate on KanAmp Product is pBca9145-I716225.