Digestion Products
|
Number
| Product Name
| Matrix Name
| Enzyme 1
| Enzyme 2
| Size
| Description
| Product Left
| Date
|
D1
| pJ23100
| pJ23100
| Spe1
| Pst1
| 2096
| Vector with the J23100 promoter ready for insertion of a RBS/CDS insert
|
| July 17
|
D2
| mRFP
| pJ23100
| Spe1
| Pst1
| 887
|
|
| July 17
|
D3
| pJ23107
| pJ23107
| Spe1
| Pst1
| 2096
| Vector with the J23107 promoter ready for insertion of a RBS/CDS insert
|
| July 17
|
D4
| mRFP
| pJ23107
| Spe1
| Pst1
| 887
|
|
| July 17
|
D5
| pJ23100
| pJ23100
| EcoR1
| Spe1
| 2925
| The vector with the mRFP gene ready for insertion of a promoter. We are not sure the digestion worked properly.
|
| July 17
|
D6
| pJ23107
| pJ23107
| EcoR1
| Spe1
| 2925
| The vector with the mRFP gene ready for insertion of a promoter. We are not sure the digestion worked properly.
|
| July 17
|
D7
| Lox66 DapA E.coli
| Lox66 DapA E.coli (PCR product)
| EcoR1
| Pst1
| 977
|
|
| July 17
|
D8
| Lox66 DapA E.coli
| Lox66 DapA E.coli (PCR product)
| Xba1
| Pst1
| 962
|
|
| July 17
|
D9
| pJ23100 ES vector
| pJ23100 (MP1.1 & MP1.2)
| EcoI
| SpeI
| 2925
| Vector with mRFP RBS/CDS ready for insertion promoter (fw-insert)
|
| July 21
|
D10
| pJ23100 promoter fw-insert
| pJ23100 (MP1.1 & MP1.2)
| EcoI
| SpeI
| 58
| J23100 promoter ready for use as a fw-insert
|
| July 21
|
D11
| pJ23107 ES vector
| pJ23107 (MP2.1, MP2.2, MP2.3 & MP2.4)
| EcoRI
| SpeI
| 2925
| Vector with mRFP RBS/CDS ready for insertion of a fw-insert promoter
|
| July 21
|
D12
| pJ23107 promoter fw-insert
| pJ23107 (MP2.1, MP2.2, MP2.3 & MP2.4)
| EcoRI
| SpeI
| 58
| J23107 promoter ready for usage as a fw-insert
|
| July 21
|
D13
| mRFP
| pJ23107 (MP2.2, MP2.3 & MP2.4)
| SpeI
| PstI
| 887
| mRFP RBS/CDS/T sequence, can it be used as a backward insert? => yes but you will not be able to add any biobrick after it
|
| July 21
|
D14
| Cre ORF
| J61047 MP9.1 & MP9.2
| XbaI
| PstI
| 1063
| Cre ORF extracted for usage as a bw-insert
| Ø D14.1
| July 21
|
D15
| B0030 bw-vector
| B0030 MP5.1 & MP5.2
| SpeI
| PstI
| 2076
| bacward vector for cloning a CDS bw-insert after the B0030 RBS
|
| July 21
|
D18
| BB dig. lox66DapAE.coli
| lox66-DapAE.coli P5 PCR product
| EcoRI
| PstI
| ~960
| PCR product digested for cloning as a BioBrick
| Ø
| July 21
|
D19
| BB dig. lox66DapAsubtilis
| lox66-DapAsubtilis P6 PCR product
| EcoRI
| PstI
| ~960
| PCR product digested for cloning as a BioBrick
| Ø
| July 21
|
D19'
| BB dig. lox66DapAsubtilis
| lox66-DapAsubtilis P6' PCR product
| EcoRI
| PstI
| ~960
| PCR product digested for cloning as a BioBrick
|
| July 30
|
D20
| bw-insert lox66-DapAE.coli
| lox66-DapAE.coli P5 PCR product
| XbaI
| PstI
| ~960
| PCR product digested for usage as a backward insert
| Ø
| July 21
|
D21
| bw-insert lox66-DapAsubtilis
| lox66-DapAsubtilis P6 PCR product
| XbaI
| PstI
| ~960
| PCR product digested for usage as a backward insert
| Ø
| July 21
|
D21'
| bw-insert lox66-DapAsubtilis
| lox66-DapAsubtilis P6' PCR product
| XbaI
| PstI
| ~960
| PCR product digested for cloning as a BI
|
| July 30
|
D22
| pSB1A2 open vector
| BBa_J61047 (MP9.1 & MP9.2)
| EcoI
| PstI
| Ø D22.1
| Open pSB1A2 vector for BioBrick EcoRI/PstI insertio
| D22.1 Ø
| July 23
|
D23
| AraC/pBad promoter fw-insert
| BBa_I0500 (MP4.1 & MP4.2)
| EcoI
| SpeI
| 1233
| AraC/pBad promoter ready for use as a forward insert
| Ø D23.1
| July 23
|
D24
| eCFP bw-insert
| BBa_E0422 (MP6.1 & MP6.2)
| XbaI
| PstI
|
| eCFP (RBS-OFR_LVA-T) ready for use as a backward insert
|
| July 23
|
D25
| PoPs to GFP converter
| BBa_E0421 (MP7.1 & MP7.2)
| XbaI
| PstI
|
| PoPs to GFP converter ready for use as a backward insert
|
| July 23
|
D26
| gfp-tripart
| BBa_E0840 (MP8.1 & MP8.2)
| XbaI
| PstI
|
| gfp-tri part (strongRBS-ORF-T) ready for use as a backward insert
|
| July 23
|
D27
| lox71-ftsZ bw-insert
| lox71-ftsZ PCR product P7
| XbaI
| PstI
| lox71-ftsZ(unmutated) ready for use as a backward insert
|
| Ø
| July 23
|
D27'
| lox71-ftsZ bw-insert
| lox71-ftsZ PCR product P7
| XbaI
| PstI
|
| lox71-ftsZ(unmutated) ready for use as a backward insert
|
| July 30
|
D28
| RBS-DapAColi BB
| P8 (RBS-DapAColi)
| EcoRI
| PstI
| ~950
| Biobrick
|
| July 25
|
D29
| RBS-DapAColi
| P8 (RBS-DapAColi)
| XbaI
| PstI
| ~950
| Backward insert
|
| July 25
|
D30
| RBS-DapASubtilis BB
| P9 (RBS-DapASubtilis)
| EcoRI
| PstI
| ~950
| Biobrick
|
| July 25
|
D31
| RBS-DapASubtilis
| P9 (RBS-DapASubtilis)
| XbaI
| PstI
| ~950
| Backward insert
|
| July 25
|
D32
| Lox71-FtsA-FtsZ
| P10 Lox71-FtsA-FtsZ
| XbaI
| PstI
| ~2600
| Backward insert - EcoRI site still presents : we can't use EcoRI
|
| July 25
|
D33
| RBS(B0030)CreORF
| L6' (clone 1 & 2)
| XbaI
| PstI
|
| RBS-Cre digestion for use as a BI
|
| July 30
|
D34
| lox66
| L9' lox66
| SpeI
| PstI
|
| Biobrick plasmid carrying lox66 digested as a BV
|
| July 30
|
D35
| lox71
| L10' lox71
| SpeI
| PstI
|
| Biobrick plasmid carrying lox71 digested as a BV
|
| July 30
|