Saint Petersburg

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Saint-Petersburg iGem2007 team

This year our team is participating for the first time in iGem2007 project.

Our team works at the [http://www.spbstu.ru Saint-Petersburg State Polytechnical University] in the department of Biophysics.

About Us

Our Project

General

The aim of our project is to build on the E.Coli bacteria the copper sensor. To solve this problem we need to use the copper-homeostasis system of E.Coli. The most convenient for our purposes protein is CusR, the component of the Copper-Responsive Two-Component System.

We intend to use the CusR promoter with the reporter gene to build sensor.

Trigger with two copper-depended promoters

So we perform a theoretical study of this problem.

So, if we want to use only proteins without secondary metabolites, the best trigger sheme is the next:

In this scheme we can observe the switching between two repressors (x and y), depending on the transcription level from the copper-depended promoter.

But this scheme is rather difficult and need many constructing operations. So, we decided to think more simple scheme, using the secondary metabolites.