From 2007.igem.org

The Missouri Western team has proposed to build this graph, which has an edge from node 1 to 2, 2 to 3, and 1 to 3. In order to determine the likelihood that the biology should find the Hamiltonian path within this directed graph, Western has looked at starting arrangements which should be identified as easy, medium, and hard.

For the time being, we shall identify an easy starting arrangement as one which, with one flip, finds a true postive. Similarily, a medium starting arrangement should find a true positive within 2 flips and and a hard should find a true positive within 3 flips.