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Growth kinetics of w121 strain

We make an array to test growth of w121 on different growth media (LB, S0.2, S0.4, S0.6, S0.8), supplemented with different amount of DAP.

Kinetic Array :le titre ici
1 2 3 4 5 6 7 8 9 10 11 12
A H20 H20 H20 H20 H20 H20 H20 H20 H20 H20 H20 H20
B H20 LB+0µM DAP LB+13µM DAP LB+17µM DAP LB+20µM DAP LB+23µM DAP LB+27µM DAP LB+30µM DAP LB+33µM DAP LB+37µM DAP LB+40µM DAP H20
C H20 S0.2+0µM DAP S0.2+13µM DAP S0.2+17µM DAP S0.2+20µM DAP S0.2+23µM DAP S0.2+27µM DAP S0.2+30µM DAP S0.2+33µM DAP S0.2+37µM DAP S0.2+40µM DAP H20
D H20 S0.4+0µM DAP S0.4+13µM DAP S0.4+17µM DAP S0.4+20µM DAP S0.4+23µM DAP S0.4+27µM DAP S0.4+30µM DAP S0.4+33µM DAP S0.4+37µM DAP S0.4+40µM DAP H20
E H20 S0.4+0µM DAP S0.4+13µM DAP S0.4+17µM DAP S0.4+20µM DAP S0.4+23µM DAP S0.4+27µM DAP S0.4+30µM DAP S0.4+33µM DAP S0.4+37µM DAP S0.4+40µM DAP H20
F H20 S0.6+0µM DAP S0.6+13µM DAP S0.6+17µM DAP S0.6+20µM DAP S0.6+23µM DAP S0.6+27µM DAP S0.6+30µM DAP S0.6+33µM DAP S0.6+37µM DAP S0.6+40µM DAP H20
G H20 S0.8+0µM DAP S0.8+13µM DAP S0.8+17µM DAP S0.8+20µM DAP S0.8+23µM DAP S0.8+27µM DAP S0.8+30µM DAP S0.8+33µM DAP S0.8+37µM DAP S0.8+40µM DAP H20
H H20 H20 H20 H20 H20 H20 H20 H20 H20 H20 H20 H20

The 96 plates is surrounded by H2O in order to maintain humidity rate during acquisition of the kinetic. In each slot, 200µL of the growth medium (LB or S0.x) is mixed with 2µL of w121 culture grown ON, and with different amount of DAP (see table). The kinetic is measured during 20H, one point is acquired each 4min10s.