Alberta/Calender/July

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(Difference between revisions)
(July 9)
(July 8)
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== July 8 ==
== July 8 ==
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'''Lab Notes:'''
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Transform E1010 and A0500 into XL10 Gold. Plate on Kan.
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Pour Kan and Amp plates.  Make TBE and TAE buffer.
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-ED, JP, MC, VH, CZ
[[Alberta/Calender/July#July|to the top]]
[[Alberta/Calender/July#July|to the top]]

Revision as of 16:08, 12 July 2007

Contents

July

July 2007
Su M Tu W Th F Sa
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31


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July 1

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July 2

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July 3

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July 4

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July 5

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July 6

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July 7

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July 8

Lab Notes:

Transform E1010 and A0500 into XL10 Gold. Plate on Kan.

Pour Kan and Amp plates. Make TBE and TAE buffer.

-ED, JP, MC, VH, CZ

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July 9

Lab Notes:

Retransform E1010 and A0050 into XL10Gold cells using new plates that were poured Sunday.

-ED, VH, JG

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July 10

Lab Notes:

Transformations of E1010 and A0050 were not successful. Tomorrow we will try with a new cell line and enriched media.

-ED, MC, AF, CZ

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July 11

Lab Notes:

Third attempt at transforming the Kan resistant BioBricks. Transformed E1010, A0500, and pSB2K3 into XL10 Gold, XL10 Gold with enriched media, and DH5a cells. Plated 100uL and the pellet remaining after taking the 100uL aliquot on Kan plates.

Restriction digest of J61003 with Xba and Spe. Run on 0.8% agarose gel. Cut out 2290 band, will gel extract tomorrow.

-ED, MC, NG, JG, VH, CZ

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July 12

General Notes:

Calender up and running.

Meeting tonight at 7:00 in CSC 2-49.

Lab Notes:

Transformations with DH5a cells worked, but we will need to select for single colonies tonight.

-ED

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July 13

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July 14

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July 15

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July 16

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July 17

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July 18

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July 19

Meeting at 7:00, Room TBA.


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July 20

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July 21

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July 22

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July 23

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July 24

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July 25

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July 26

Meeting at 7:00, Room TBA.


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July 27

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July 28

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July 29

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July 30

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July 31

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