(Difference between revisions)
(September 9)
(September 9)
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NG - Update: 9:30am 'broke' into lab :D.  Then found key...
NG - Update: 9:30am 'broke' into lab :D.  Then found key...
11-2: VH, CZ
11-2: VH, CZ<br>
Nick ran the gel of I0500 restricited by Eco/Spe, but nothing showed up as expected (1.5kb). <br>
Digested I0500 and J61003 with Eco/Pst as instructed.<br>
2-5: MC, JG<br>
2-5: MC, JG<br>

Revision as of 03:13, 10 September 2007



September 2007
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September 1

Lab Notes
1. 24 Minipreps for Diezel Blaze in B0034 using Fermentas Kit; stored in -20 freezer
2. Lots of colonies from the transformation last night; no colonies on negation control (good) and lots of colonies on positive control (good) and no satellite colonies (also good); no colonies were picked for overnights
3. Run the Buddy+Benny in B00 and Betty+Enny in B00 Pst/Xba gel from last night at 50V for another 30 minutes for better resolution; all the digest worked so I cut out the appropriate bands and stored the gel in -20 freezer for purification later


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September 2

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September 3

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September 4


JP,ML,JG 7:00pm

For the record:
Buddy = BuOH DH 1235 bp
Betty = Bb-bhBu-coa dh 923 bp
Benny = Butyryl coa dh 1184 bp
enny = Enoyl coa Hydratase 860 bp
Diezel Blaze = buAld-Dh 2639 bp

Moving was done this mid-day.
New location M349
JG has key & access
- MC, ED, JG

Evening crew - please do a once over of G308 to double check we have not left anything and to clean up anything extra/ things we should get out of the way. thnx - MC

Night Crew


Set up new lab

Things we need - Water Bath, Scale, disposable 13ml overnight tubes, Tip waste

lab work

Gel purification ran on Betty,enny and boo as well as buddy and benny and is in the -20 in the new lab

To Do list for tomorrow

- Restriction on DB needs to be done - If we want to put it in front of BE or BB then Spe/Pst - If we want to put it behind BE or BB then cut with Xba/Pst - maybe do both (use 4microL DNA for digest)

-complete restriction on BE and BB for SPE and PST (then we can complete ligations BE-BB and BB-BE or we can ligate D.B into BE or BB to make BE-DB or DB-BE or BB-DB or DB-BB- Then we can ligate BE-BB-DB or DB-BE-BB or BE-BB-DB or BB-DB-BE or BB-BE-DB and we are kinda finished!

- If I0500 comes in from calgary it needs to be transformed.

- Transform R0080 which is a back up promoter just in case?

-Note we also have to do sequenceing on BB and BE and DB

Shout out to calgary for saving our necks Shout out to the move in crew to the top

September 5


NG,ED,ML 7:00pm

AM Crew Notes:

finished up cleaning up post-move in
got a 'water bath' - JG calibrated it
prepared BB in Boo & BE in Boo for sequencing - this will be ready for pick up tomorrow
- DB in BOO with PST/XBA
- DB in BOO with PST/SPE
- BB in BOO with PST/SPE
- BE in BOO with PST/SPE
NB: there is a section in the masterbook "figuring stuff out" just a few questions I have from being away so long - don't worry about it we should do a wet lab recap to bring everyone on the same page at our meeting Thrusday - MC
<3 JG & MC

Transformed I0500 from Calgary. Transformed R0080. Ran digestions on gel. Ligated digested DB with BB and BE. (all in Boo)


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September 6


NK ED AL 5:00pm (Unless your crazy enough to show up at 5AM)

To Do:

Goodies will be served during the meeting (7:00pm @ CAB 373), so be sure to show up!

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September 7


AM Crew: MC, JG meeting at 800hrs

Lab Notes:
No growth observed for R0080 overnights; just scrapping it because we have I0500
No growth on transformations of BB+DB and BE+DB ligations therefore . . . religated - see masterbook for more details
O/N with AMP for I0500
Transform religations in to XL10 Gold

NB: JG has key access

To Do:
Mini preps on I0500 overnights

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September 8


Super Awesome Marathon of Productiveness

8-11: JG,NG

11-2: AL,ML

2-5: VH,NK

5-8: JP

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September 9


And Yet another super productive Sunday

8-11: NG and CZ

NG - Nobody Called me about the key last night (and I completely forgot to swing by: was with parents). So I will wait by the lab either , until the next group shows up or someone with a key. Also bioSci is locked but there is an open door by the physcology wing. I have my cell on 902-8032.

NG - Update: 9:30am 'broke' into lab :D. Then found key...

11-2: VH, CZ
Nick ran the gel of I0500 restricited by Eco/Spe, but nothing showed up as expected (1.5kb).
Digested I0500 and J61003 with Eco/Pst as instructed.

2-5: MC, JG
ran gel of restricted I0500 & J61003; unfortunately there is no DNA in the I0500 sample either
gel extracted J61003
Made LB - to be divided into bottles & autoclaved in G308
O/N of DB in BB & DB in BE samples left in 37degree room

5-8: ML, ED

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September 10

Schedule: And Scheduling just got hard core

I have sheduled what we have taken down in the meeting some people appear many times on this schedule working alone just because of the time they were availible. If you are comfortable working alone and taking on lots of shifts im sure the team would be greatful but I'm also sure we would also understand if you didn't want to work alone in the lab. I have only scheduled this way because we need man hours so i tried to fill up all the spots as best i could. -JG

MC,JG for the AM

ML,AL for the MID

NG for the PM (celine is in calgary if you dont feel comfortable in the lab by yourself I can show up - JG)

JP,ED for the Night

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September 11

Schedule: No one else really signed up for tuesdays so anyone who has time to drop in should


Mid - AL

Night- ED JP

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September 12


AM - MC Sorry this is one of the "odd" wednesdays i am unable to make it in the AM until 1130hrs. . . Or i can come in from 800hrs to 920ish. . . but then i have go jet for 2hrs

Mid- ML, AL


Night - CZ, ED

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September 13




Meeting 7:00

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September 14



Mid - AL


night - JP

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September 15


And yet another crazy weekend...

8-11 - ED,MC

11-2 - JG, ML

2-5 - NG, CZ

5-8 - NG, ( this is what i had copied down but if anyone else can take this im sure nick would be greatful)

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September 16

Schedule: Continued...

8-11 ED, JG

11-2 VH, CZ

2-5 MC, JP

5-8 - ML, NG

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September 17

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September 18

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September 19

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September 20

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September 21

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September 22

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September 23

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September 24

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September 25

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September 26

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September 27

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September 28

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September 29

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September 30

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