Berkeley LBL/JoyceNotebook

(Difference between revisions)

Revision as of 19:02, 25 October 2007

Contruction of pET3a Derivatives Containing bchD and bchI

1. Amplify Rhodobacter's gene and introduce restriction sites, which allow cloning PCR fragments into pET3a

	bchD	bchI
Restriction sites introduced when amplified by PCR	NdeI, BamHI	NdeI, BglI

@@ Line 1: / Line 1: @@
-'''Contruction of pET3a Derivatives Containing bchH and bchI'''
+'''Contruction of pET3a Derivatives Containing ''bchD'' and ''bchI'''''
+. Amplify Rhodobacter's gene and introduce restriction sites, which allow cloning PCR fragments into pET3a
+{| border =
+|-
+!
+!''bchD''
+!''bchI''
+|-
+|Restriction sites introduced when amplified by PCR
+|NdeI, BamHI
+|NdeI, BglI
+|}