Berkeley LBL/JoyceNotebook

From 2007.igem.org

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!''bchD''  
!''bchD''  
!''bchI''
!''bchI''
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|length of gene fragment
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|1695 b.p.
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|1005 b.p.
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|NdeI, BamHI
|NdeI, BamHI
|NdeI, BglI
|NdeI, BglI
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Revision as of 19:26, 25 October 2007

Contruction of pET3a Derivatives Containing bchD and bchI

1. Amplify Rhodobacter's gene and introduce restriction sites by PCR (Using Phusion Polymerase), which allow cloning PCR fragments into pET3a

bchD bchI
length of gene fragment 1695 b.p. 1005 b.p.
Restriction sites introduced when amplified by PCR NdeI, BamHI NdeI, BglI