Berkeley LBL/Laina Notebook
From 2007.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
Biobricking of crtN | Biobricking of crtN | ||
| - | [[ | + | GenecrtN |
| + | [[gene]] | ||
PCR of crtN on Heliobacillus mobilis | PCR of crtN on Heliobacillus mobilis | ||
| Line 12: | Line 13: | ||
crtN-F cloning of crtN 5’ccaaaAGATCTgtgaaacatacagcaaaaaacctgggt3’ | crtN-F cloning of crtN 5’ccaaaAGATCTgtgaaacatacagcaaaaaacctgggt3’ | ||
| + | |||
crtN-R cloning of crtN 5’gctagCTCGAGttaGGATCCtcagtaactggctgacaagcct3’ | crtN-R cloning of crtN 5’gctagCTCGAGttaGGATCCtcagtaactggctgacaagcct3’ | ||
Revision as of 04:02, 27 October 2007
Biobricking of crtN
GenecrtN gene
PCR of crtN on Heliobacillus mobilis G/C content on annealing region
crtN-F 40% BglII CrtN-R 50% BamHI and XhoI
Primers
crtN-F cloning of crtN 5’ccaaaAGATCTgtgaaacatacagcaaaaaacctgggt3’
crtN-R cloning of crtN 5’gctagCTCGAGttaGGATCCtcagtaactggctgacaagcct3’
