Berkeley LBL/Laina Notebook
From 2007.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | Biobricking of | + | Biobricking of bch-gene cluster and crtN gene. |
Gene: | Gene: | ||
| - | crtN [[gene]] | + | {| border="0" cellspacing="8px" cellpadding="15" width="80%" |
| + | |[[bchB]] | ||
| + | |[[bchE]] | ||
| + | |[[bchI]] | ||
| + | |[[bchL]] | ||
| + | |[[bchM]] | ||
| + | |[[bchN]] | ||
| + | |[[crtN[[gene]]]] | ||
| + | |} | ||
| + | crtN | ||
PCR of crtN on Heliobacillus mobilis | PCR of crtN on Heliobacillus mobilis | ||
Revision as of 04:28, 27 October 2007
Biobricking of bch-gene cluster and crtN gene.
Gene:
| bchB | bchE | bchI | bchL | bchM | bchN | [[crtNgene]] |
crtN
PCR of crtN on Heliobacillus mobilis G/C content on annealing region
crtN-F 40% BglII CrtN-R 50% BamHI and XhoI
Primers
crtN-F cloning of crtN 5’ccaaaAGATCTgtgaaacatacagcaaaaaacctgggt3’
crtN-R cloning of crtN 5’gctagCTCGAGttaGGATCCtcagtaactggctgacaagcct3’
