Berkeley LBL/Laina Notebook

From 2007.igem.org

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'''Materials'''  
'''Materials'''  
'''Methods'''  
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Biobricking of bch-gene cluster and crtN gene.
Gene:
Gene:
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'''Results'''  
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'''Discussion'''Biobricking of bch-gene cluster and crtN gene.
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'''Discussion'''

Revision as of 05:02, 27 October 2007

Materials

Methods

Biobricking of bch-gene cluster and crtN gene.

Gene:

bchB bchE bchI bchL bchM bchN [[crtNgene]]

crtN

PCR of crtN on Heliobacillus mobilis G/C content on annealing region

crtN-F 40% BglII CrtN-R 50% BamHI and XhoI

Primers

crtN-F 3’ccaaaAGATCTgtgaaacatacagcaaaaaacctgggt5’

crtN-R 5’gctagCTCGAGttaGGATCCtcagtaactggctgacaagcct3’

bch B 3'ttaGGATCCccgttcgccttggtttgacttact5'

Results

Discussion