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	'''Methods'''		'''Methods'''
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		+	PCR
		+	Dephosphorylation
		+	Sequencing
	Biobricking of bch-gene cluster and crtN gene.		Biobricking of bch-gene cluster and crtN gene.

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Revision as of 05:05, 27 October 2007

Materials

Methods

PCR Dephosphorylation Sequencing

Biobricking of bch-gene cluster and crtN gene.

Gene:

bchB

bchE

bchI

bchL

bchM

bchN

[[crtNgene]]

crtN

PCR of crtN on Heliobacillus mobilis G/C content on annealing region

crtN-F 40% BglII CrtN-R 50% BamHI and XhoI

Primers

crtN-F 3’ccaaaAGATCTgtgaaacatacagcaaaaaacctgggt5’

crtN-R 5’gctagCTCGAGttaGGATCCtcagtaactggctgacaagcct3’

bch B 3'ttaGGATCCccgttcgccttggtttgacttact5'

Results

Discussion