Berkeley LBL/Laina Notebook

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Revision as of 04:32, 27 October 2007 by Silvapy (Talk | contribs)

Biobricking of bch-gene cluster and crtN gene.

Gene:

bchB bchE bchI bchL bchM bchN [[crtNgene]]

crtN

PCR of crtN on Heliobacillus mobilis G/C content on annealing region

crtN-F 40% BglII CrtN-R 50% BamHI and XhoI

Primers

crtN-F cloning of crtN 5’ccaaaAGATCTgtgaaacatacagcaaaaaacctgggt3’

crtN-R cloning of crtN 5’gctagCTCGAGttaGGATCCtcagtaactggctgacaagcct3’ bch B|3'ttaGGATCCccgttcgccttggtttgacttact