Berkeley LBL/Ligation
From 2007.igem.org
< Berkeley LBL(Difference between revisions)
Latest revision as of 21:12, 17 October 2007
Ligation (20 uL scale):
1. Create ligation mixture:
12 uL backbone
4 uL insert
2 uL Ligase Buffer
1 uL Ligase Enzyme
1 uL H2O
2. Allow to ligate at room temperature for at least 1 hour