Berkeley LBL/Ligation

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Revision as of 21:12, 17 October 2007 by Joyxi (Talk | contribs)
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Ligation (20 uL scale):

1. Create ligation mixture:

     12 uL backbone
     4 uL insert
     2 uL Ligase Buffer
     1 uL Ligase Enzyme
     1 uL H2O


2. Allow to ligate at room temperature for at least 1 hour