From 2007.igem.org

Let's make I716008 (Ptet-rbs-wbbL-rbs-neuS)
[digest] pcr prod of Ptet (EcoRI/BamHI, 69+6, L)
[digest] pBca1122-Bca9270 (EcoRI/BglII, 1027+4140, L)
[ligate]
[prod] I716008 (Ptet-rbs-wbbL-rbs-neuS)


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(pcr prod) Ptet gaattcatgAGATCTtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacGGATCC