BerkiGEM2007-AustinConstructionFile18
From 2007.igem.org
(Difference between revisions)
Revision as of 00:21, 22 June 2007
PCR AD0005F/AD0005R on 9145-I716062 -HbA alpha 2X mutant-
PCR AD0006F/AD0006R on 9145-I716062 –HbA alpha 2X mutant-
Digest with BsaI and Ligate, product name is ADtemp1
PCR AD0005F/AD0006R on ligation ADtemp1
Digest ADtemp1 (EcoR1/Xho1, 870/28, larger)
Sub into 9145-anything
Product is 9145-I716068
