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-	PCR AD0005F/AD0005R on 9145-I716062 -HbA alpha 2X mutant- <br>	+	PCR ca1099F/AD0005R on 9145-I716062 -HbA alpha 2X mutant- (1445 bp)<br>
-	PCR AD0006F/AD0006R on 9145-I716062 –HbA alpha 2X mutant-<br>	+	PCR AD0006F/ca1099R on 9145-I716062 –HbA alpha 2X mutant- (1577 bp)<br>
-	Digest with BsaI and Ligate, product name is ADtemp1<br>	+	Digest both with BsaI/DpnI, and Ligate together
-	PCR AD0005F/AD0006R on ligation ADtemp1<br>	+
-	Digest ADtemp1 (EcoR1/Xho1, 870/28, larger)<br>	+
-	Sub into 9145-anything<br>	+
	Product is 9145-I716068<br>		Product is 9145-I716068<br>

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Latest revision as of 21:52, 26 June 2007

PCR ca1099F/AD0005R on 9145-I716062 -HbA alpha 2X mutant- (1445 bp)
PCR AD0006F/ca1099R on 9145-I716062 –HbA alpha 2X mutant- (1577 bp)
Digest both with BsaI/DpnI, and Ligate together Product is 9145-I716068