BerkiGEM2007-AustinConstructionFile18
From 2007.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | PCR ca1099F/AD0005R on 9145-I716062 -HbA alpha 2X mutant- <br> | + | PCR ca1099F/AD0005R on 9145-I716062 -HbA alpha 2X mutant- (1445 bp)<br> |
| - | PCR AD0006F/ca1099R on 9145-I716062 –HbA alpha 2X mutant-<br> | + | PCR AD0006F/ca1099R on 9145-I716062 –HbA alpha 2X mutant- (1577 bp)<br> |
Digest both with BsaI/DpnI, and Ligate together | Digest both with BsaI/DpnI, and Ligate together | ||
Product is 9145-I716068<br> | Product is 9145-I716068<br> | ||
Latest revision as of 21:52, 26 June 2007
PCR ca1099F/AD0005R on 9145-I716062 -HbA alpha 2X mutant- (1445 bp)
PCR AD0006F/ca1099R on 9145-I716062 –HbA alpha 2X mutant- (1577 bp)
Digest both with BsaI/DpnI, and Ligate together
Product is 9145-I716068
