BerkiGEM2007-AustinConstructionFile18

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PCR AD0005F/AD0005R on 9145-I716062 -HbA alpha 2X mutant-
PCR AD0006F/AD0006R on 9145-I716062 –HbA alpha 2X mutant-
Digest with BsaI and Ligate, product name is ADtemp1
PCR AD0005F/AD0006R on ligation ADtemp1
Digest ADtemp1 (EcoR1/Xho1, 870/28, larger)
Sub into 9145-anything
Product is 9145-I716068