p-TRC (Bgl2/Xho1, 4136/1713, Larger) I716067 -Cmr-rbs-beta-rbs-alpha (Bgl2/Xho1, 2949/970, Smaller) Ligated Product is I716069 2X mutant -pTRC-rbs-beta-rbs-alpha (Select the [hopefully] bloodly looking colonies)