BerkiGEM2007-AustinConstructionFile19

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p-TRC (Bgl2/Xho1, 4136/1713, Larger)
I716067 -Kan-rbs-beta-rbs-alpha (Bgl2/Xho1, 3081/970, Smaller)
Ligated Product is I716069 2X mutant -pTRC-rbs-beta-rbs-alpha (Select the [hopefully] bloodly looking colonies)